Fig. 5: Germinal centers of tumor-draining lymph nodes. | Nature Immunology

Fig. 5: Germinal centers of tumor-draining lymph nodes.

From: Simultaneous STING and lymphotoxin-β receptor activation induces B cell responses in tertiary lymphoid structures to potentiate antitumor immunity

Fig. 5

Tumor-draining lymph nodes were collected on day 14 and frozen sections examined by immunofluorescence of lymphoid follicles. a, Staining of lymph node follicles of combination therapy-treated and untreated control mouse groups with CD19 (magenta) and CD69 (green) antibodies to assess the activation of follicular B cells in these lymph nodes. Representative sections of six lymph nodes are shown for each group. Scale bars, 100 μm. b, Similarly, staining with CD19 (magenta) and CD73 (green) antibodies to detect memory B cells in the lymph node follicles of combination therapy-treated and untreated control mice. Scale bars, 100 μm. c, Clusters of Ki-67+ B cells within CD19+ B cell follicles were recognized as germinal centers (GCs) by immunostaining of each lymph node section. The number of GCs (left) and the total area of GCs (right) in each lymph node (LN) section are presented in the graphs. Inguinal lymph nodes of mice without tumor implantation were used for intact lymph node control. N = 6, *P < 0.05, **P < 0.01.

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