Fig. 7: Single-cell RNA-seq analyses of tumor-infiltrating leukocytes.

Tumor-infiltrating CD45⁺ cells were analyzed on day 14 by single-cell RNA-seq following FACS sorting. a, Left: uniform manifold approximation and projection (UMAP) of different CD45⁺ leukocyte populations was generated using sets of genes for cell-type annotations as listed in Extended Data Fig. 9. Right: relative abundance of different immune cell types is shown as the fractions of total tumor-infiltrating CD45⁺ leukocytes in indicated treatment groups. b, Each B cell subtype was identified by expression of indicated marker genes. PC, plasma cell; LPC, long-lived plasma cell. c, Upper plots: the abundance of intratumoral B cell subtypes in different treatment groups. Lower plots: the abundance of intratumoral B cell populations expressing different isotypes of immunoglobulins. d–f, Intratumoral CD4⁺ T cell subtypes (naive, T_H1, T_H2 and T_H17) are shown as separate clusters in the t-SNE plots of different treatment groups (d). The identity of each T cell subtype was determined by the expression of indicated marker genes (e). The relative abundance of CD4⁺ T cell subtypes is shown as the fraction of total intratumoral CD4⁺ T cells in different treatment groups (f). DC, dendritic cell.