Extended Data Fig. 5: DNA methylation tracing and editing of SMRs in ILCPs.
a, Representative flow cytometry plots showing GFP expression in Lin−CD127+c-Kit+α4β7+PD-1+ ILCPs transduced with lentivirus carrying Dazl-CGI-Snrpn-GFP methylation reporter systems. The GFP expression in mCherry+ ILCPs was measured two days after transduction. b, Barplots showing proportions of GFP+ ILCPs among mCherry+ ILCPs expressing Tbx21-SMR-Snrpn-GFP (Tbx21), Bcl11b-SMR-Snrpn-GFP (Bcl11b), and Rorc-SMR-Snrpn-GFP (Rorc) reporter systems two days after lentiviral transduction. Data are shown as mean ± s.d. (n = 4 cultures per group). c, Boxplot showing DNA methylation levels of SMRs after DNA methylation editing in ILCPs. Lin−CD127+c-Kit+α4β7+PD-1+ ILCPs were transduced with lentiviruses encoding either active dCas9-DNMT1 (dC-DNMT1) or a catalytically inactive mutant (dC-dDNMT1), along with SMR-targeting gRNAs. The DNA methylation levels of SMRs were measured by bisulfite sequencing and shown. Each box represents the median and the 25% and 75% quartiles, and the whiskers indicate 1.5 times the interquartile range. Statistical analyses were performed using two-tailed unpaired Student’s t test. (n = 10 per group). Data are representative of at least three independent experiments.
