Extended Data Fig. 7: Dnmt1 deletion impairs ILCP differentiation.

a, Bar graph showing the proportions of UHRF1-mCherry⁺ and UHRF1-mCherry⁻ ILCPs in the bone marrow of Id2^CreERT2 UHRF1-mCherry mice or Id2^CreERT2Dnmt1^fl/fl UHRF1-mCherry mice after TMX treatment. Data were shown as mean ± s.d. (n = 3 mice per group). b, Representative flow cytometry histograms (left) showing the expression levels of UHRF1-mCherry in Id2^CreERT2Dnmt1^fl/fl UHRF1-mCherry ILCPs in the cell culture with OP9-DL1 cells with 25 ng/ml IL-7 and 25 ng/ml SCF at the initial time point (green line), two days later with (yellow line) or without 4-OHT treatment (red line). ILCPs from WT mice served as a control (grey line). The MFI of UHRF1-mCherry was analyzed by flow cytometry and shown as mean ± s.d (right). Statistical analyses were performed using two-tailed unpaired Student’s t test. (n = 3 cultures per group). c, Principal component analysis (PCA) showing single-cell clustering based on DNA methylation levels in promoters, colored by the indicated cell type. UHRF1-mCherry⁺ ILCPs (ILCP1s) from Id2^CreERT2Dnmt1^fl/fl UHRF1-mCherry⁺ mice treated with TMX (n = 26 cells) were isolated for single-cell multiomics sequencing and clustered with ILCP1s (n = 106 cells) and ILCP2s (n = 206 cells). Data are representative of at least two (c) or three (a, b) independent experiments.

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