Extended Data Fig. 6: Disease stroma profiles. | Nature Immunology

Extended Data Fig. 6: Disease stroma profiles.

From: A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues

Extended Data Fig. 6

a) Fibroblast proportions in neurofibroma and dotplot of expression of F2/3 and F5 marker genes for fibroblasts in neurofibroma. b) Methodology for random forest classifier. Results: F1-score for scarring risk category classification and importance scores by fibroblast subtype. c) Heatmap of marker genes for fibroblast and non-fibroblast populations in Xenium 5k data for lesional/inflamed atopic dermatitis skin. d) Cell2location predictions and histopathological annotation for lesional (inflamed) atopic dermatitis skin profiled by 10x Visium. Same scale used for fibroblast subtypes as shown in Extended Data Fig. 3a. e,f) Zoomed in regions of perivascular inflammatory infiltrate (predicted location of F3: FRC-like fibroblasts). g) Heatmap of marker genes for fibroblast populations in Xenium 5k data for cutaneous melanoma. CAF: cancer-associated fibroblast. h) Heatmap of expression of skin CAF genes from Forsthuber et al. in our skin fibroblast subtypes (scRNA-seq data). i) Dotplot of expression of inflammatory CAF marker genes and our reported F6: Inflammatory myofibroblast marker genes in F6: Inflammatory myofibroblasts from different disease categories.

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