Extended Data Fig. 8: Additional cross-tissue populations and human skin-intestine fibroblast integration. | Nature Immunology

Extended Data Fig. 8: Additional cross-tissue populations and human skin-intestine fibroblast integration.

From: A single-cell and spatial genomics atlas of human skin fibroblasts reveals shared disease-related fibroblast subtypes across tissues

Extended Data Fig. 8

a) Heatmap of marker genes for additional reported shared/universal fibroblast populations from Gao et al. b) Proportion of fibroblasts by tissue in renamed fibroblast clusters from cross-tissue atlas. c) Dotplot of skin fibroblast marker genes for cross-tissue fibroblast clusters (excluding skin fibroblasts). Skin fibroblasts were excluded to avoid skin fibroblasts driving the gene expression signature for each cluster. d) Expression of F1 marker genes by tissue in fibroblasts annotated as F1: Superficial fibroblasts in our cross-tissue atlas. e) Dotplot of F3 marker gene expression in clustered HLCA data and bar plot showing original annotations for fibroblasts that we re-annotated as ‘F3-like’. HLCA: Human Lung Cell Atlas. f) Dotplot of skin marker genes in HLCA data (original annotations from HLCA). g) UMAP visualization for joint human skin-intestine fibroblast integration using labeled data from the gut atlas (Elmentaite et al). Dotplot of marker genes for F6: Inflammatory myofibroblasts (from skin) in F6: Inflammatory myofibroblasts (skin) and Stromal 4 (MMP1+) (intestine) populations; dotplot of marker genes for F3: FRC-like (from skin) in F3: FRC-like (skin) and T reticular cells (intestine) populations (same scale for both plots); and dotplot of marker genes for F2/3: Perivascular in F2/3: Perivascular (skin) and various intestinal populations (same scale for all plots).

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