Extended Data Fig. 7: MHC I expression across thymocyte subsets after damage.
From: Damage-induced IL-18 stimulates thymic NK cells limiting endogenous tissue regeneration
a, Female 1-2 mo C57BL/6 mice were given SL-TBI and thymuses collected 0 and 3 days later. Concatenated flow plots showing H2kb expression of early thymic progenitors (ETPs), DN2-4, DP, SP4, SP8 cDC1, cDC2 cells (see Extended Data Fig. 3 for gating) and CD45+CD11b+Tim-4+ Macrophages and their respective gMFI 0 and 3 days post SL-TBI (n = 5/group). b, Female 1-2mo C57BL/6 mice were given SL-TBI and thymuses collected 3 days later and enriched for non-hematopoietic stromal cells which were cultured with or without poly(I:C) stimulated NK cells. Annexin V and 7-AAD expression of CD45− EpCAM− MHC-II− non-TECs was measured at 5 h post co-culture (n = 4/group). c, Female 1-2 mo C57BL/6 WT (n = 5) or Il18r1−/− (n = 6) thymuses collected at baseline and Annexin V and 7-AAD expression of cTECs and mTECs assessed. d, Female 1-2 mo C57BL/6 WT (n = 10) or Il18r1−/− (n = 9) were given SL-TBI and thymuses collected at 5 days later. Annexin V and 7-AAD expression of CD45−EpCAM−MHC-II− non-TECs was measured. Graphs represent mean ± SEM; each dot represents an individual biological replicate; ns = not significant. Statistics were generated for a–d using unpaired two-tailed t-tests.
