Extended Data Fig. 1: Increased pyroptosis after distinct modalities of thymic damage.
From: Damage-induced IL-18 stimulates thymic NK cells limiting endogenous tissue regeneration
a, Thymus supernatants generated from female 1-2 mo C57BL/6 mice given SL-TBI (550 cGy), Dexamethasone (i.p., 20 mg/kg), Cyclophosphamide (i.p., 200 mg/kg) or LPS (i.p., 1.5 mg/kg) and mature Caspase-1 assayed by ELISA on days 0 (n = 4), 0.5 (n = 3), 1 (n = 3) and 3 (n = 3). b, Female 1-2 mo C57BL/6 WT (d0, n = 18; day 3, n = 15) or Il18−/− (d0, n = 9; day 3, n = 8) and Il18r1−/− (d0, n = 10; d3, n = 5) mice were given SL-TBI and thymus cellularity was assessed at baseline (day 0) or 3 days post-SL-TBI. c, Thymus supernatants generated from female 1-2 mo C57BL/6 WT (d0, n = 5; d1, n = 8; d3, n = 3) and Il18−/− (n = 4/group) mice given SL-TBI (550 cGy) and Cortisol assayed by ELISA on days 0, 1 and 3. Graphs represent mean ± SEM; each dot represents an individual biological replicate; ns = not significant. Statistics were generated for a using one-way ANOVA with Dunnet’s correction for multiple comparisons, b using Tukey’s correction for multiple comparisons, and for c using an unpaired two-tailed t-test. Panel a icons created with BioRender.com.
