Extended Data Fig. 6: Endocytosis was not required for PFO-mediated TGN remodeling or NLRP3 inflammasome activation.
a,b, Representative immunofluorescence images (a) and quantification of TGN dispersion (b) in HeLa cells transfected with V5-tagged dynamin 1 (DNM1) (WT or dominant-negative mutant K44A), dynamin 2 (DNM2) (WT or dominant-negative mutant K44A) or not (Original) before incubated with 0.90 nM rPFO or not (Mock) for 80 min. Scale bar, 5 μm. b, Areas containing TGN structures were measured with ImageJ (n = 40 cells per condition; mean ± s.d.; two-sided t-test; NS, not significant). Representative from 2 independent experiments. c, Immunoblots of HEK293T cells stably expressing NLRP3, ASC, and caspase-1 (Casp1) before transfected as in a (top) and the same cell lines incubated with 0.90 nM rPFO for 40 min (bottom). pro-Casp1 has two bands in this cell line because it was expressed as zeocinr-F2A-Casp1 (upper band) before ribosomal skipping to release pro-Casp1 (lower band). Representative from 3 independent experiments.
