Extended Data Fig. 8: Membrane repair inhibitors enhanced PFO-mediated TGN remodeling and NLRP3 recruitment.
a, Representative LDH release assay of HeLa cells pre-treated with DMSO (solvent control), 20 µM U0126, or 20 µM mirdametinib for 30 min before addition of rPFO for 80 min (n = 3 wells per condition; mean ± s.d.; two-sided t-test; NS, not significant). Representative from 3 independent experiments. b–d, Representative immunofluorescence images (b), quantification of TGN dispersion (c) and NLRP3 recruitment (d) in HeLa cells stably expressing NLRP3-GFP pre-treated with DMSO (solvent control), 20 µM U0126 or 20 µM mirdametinib for 30 min before addition of rPFO or not (Mock) for 80 min. Scale bar, 10 μm. Areas containing TGN structures were measured with ImageJ (n = 40 cells per condition). NLRP3 recruitment was quantified from 100 cells (n = 3; N.D., not detectable). Representative from 3 independent experiments.
