Extended Data Fig. 4: Validation of VirScan Z-scores by comparisons with ELISA- and Luminex-based serostatuses in the Milieu Intérieur cohort.

a,b, −log₁₀(P-values) for the association between the VirScan Z-scores and serology determined by (a) ELISA or (b) Luminex xMAP. Serology variables are plotted on the x-axis, and for each serology variable, the −log₁₀(P-values) for the association with all 2,608 public VirScan peptides are shown on the y-axis. Significant associations (FDR < 0.05) are colored in green or red if the association is or is not for the cognate virus, respectively. Non-significant associations are colored gray. c,e, −log₁₀(P-values) for the association between the VirScan Z-scores and ELISA-determined serostatus for (c) CMV and (e) IAV. Significant associations (FDR < 0.05) are colored in green or red if the association is or is not for the cognate virus, respectively. Non-significant associations are colored gray. d,f, −log₁₀(P-values) for the association between the VirScan Z-scores and Luminex-determined serology for (d) CMV and (f) IAV. Significant associations (FDR < 0.05) are colored in green or red if the association is or is not for the cognate virus, respectively. Non-significant associations are colored gray. g, VirScan Z-score distributions for the three peptides most significantly associated with ELISA-based serostatus for the EBV antigens EA, EBNA and VCA. ELISA-based serostatus for each EBV antigen is shown on the x-axis. h, Luminex-based serologies for the spike and nucleocapsid proteins shown for the four coronaviruses 229E, HKU1, NL63, and OC43.