Fig. 4: RunxWRPW redirects MHC-II-restricted thymocytes to the CD8+ T cell lineage.
a, Representative dot plots showing expression of CD4 and CD8α in CD69−TCRβ−pre-selection (pre), CD69+TCRβ+ post-selection (post) and CD24−TCRβ+ mature thymocytes and histograms showing expression of Zbtb7b in CD4−CD8+ (CD8 SPs) CD4+CD8− (CD4SPs) or CD4−CD8− (DN) thymocytes (left) and quantification of the frequency and cell numbers of CD8 SPs, CD4 SPs and DNs (right) in WT (n = 8), R26DP-R3 (n = 5) and R26DP-R3-WRPW (n = 4) mice. Data represent mean ± s.d. **P < 0.005, ***P < 0.0001, ****P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison). b, Representative dot plots showing expression of CD4 and CD8α in pre-selection (pre), post-selection (post) and mature thymocytes (left), representative zebra plots showing expression of Zbtb7b and Runx3 in CD4 SPs, CD8 SPs and DN thymocytes (middle) and quantification of the frequency and cell numbers of CD8 SPs, CD4 SPs and DNs (right) in R26DP-R1 (n = 6), R26DP-R1-WRPW (n = 8) and R264SP-DP-R1-WRPW (n = 3) mice. Data represent mean ± s.d. *P < 0.05, **P < 0.005, ***P < 0.0001, ****P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison). c, Representative dot plots showing CD4 and CD8α expression in CD24−TCRβ+ mature thymocytes (left) and quantification of frequency and cell numbers of CD4 SPs, CD8 SPs and DN thymocytes (right) from b2m−/− (n = 3), b2m−/−R26DP-R1-WRPW (n = 3), OT-II (n = 3) and OT-II R26DP-R1-WRPW (n = 4) mice. Data represent mean ± s.d. **P < 0.005, (unpaired Student’s t-test, two-sided). d, Representative dot plots showing CD4 and CD8α expression in CD24−TCRβ+ mature thymocytes (left) and quantification of frequency and cell numbers of CD4 SPs, CD8 SPs and DN thymocytes from Zbtb7bΔS/+ (n = 4), Zbtb7bΔS/+R26DP-R1-WRPW (n = 3), Tle1/3/4TKO (n = 4) and Tle1/3/4TKOR26DP-R1-WRPW (n = 3) mice. Data represent mean ± s.d. **P < 0.005, (unpaired Student’s t-test, two-sided).
