Fig. 5: Mutation of the Runx terminal Y residue influences its function.
a, Representative dot plots showing CD4 and CD8α expression in CD69+TCRβ+ post-selection (post) and CD24−TCRβ+ mature thymocytes (left) and quantification of frequency and cell numbers of mature, CD4 SPs, CD8 SPs and DP thymocytes (right) from R26DP-R1-WRPE (n = 5), R26DP-R1 (n = 5), R26DP-R1-WRPF (n = 5) and R26DP-R1-WRPW (n = 4) mice. Data represent mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison). b, Representative dot plots showing CD4 and CD8α expression in CD24−TCRβ+ mature thymocytes and TCRβ+ peripheral T cells (left) and quantification of percentage and number of CD4 SPs, CD8 SPs and DPs among mature thymocytes and peripheral T cells (right) in Rx3E/E (thymus, n = 6; spleen, n = 3), Rx3+/+ (thymus, n = 22; spleen, n = 21), Rx3F/F (thymus, n = 5; spleen, n = 6) and Rx3W/W (thymus, n = 9; spleen, n = 6) mice. Data represent mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way ANOVA with Tukey’s multiple comparison). c, Immunoblot of TLE3 antibody after immunoprecipitation of CD8 SP thymocytes from Rx3+/+ and Rx3E/E mice with an Runx3 antibody. One representative of two independent experiments. d, Quantification of the number of inguinal and axillary lymph nodes (LNs) from Rx3+/+ (n = 4), Rx3+/F (n = 4) and Rx3F/F (n = 3) mice. Data represent mean ± s.d. *P < 0.05, **P < 0.01 (one-way ANOVA with Tukey’s multiple comparison).
