Extended Data Fig. 4: Phenotypic features of Chat-GFP+ Tfh cells; related to Fig. 4.
From: Lymphocyte-derived cholinergic circuits modulate germinal center output and B cell activation
a GSEA analysis revealing enrichment for ChAT-dependent hallmark pathways in the Tfh cells in Fig. 3b. b Left: Representative flow cytometry plots of GL7+ cells, gated on Chat-GFP+ and Chat-GFP− Tfh cells at day 5 post-SRBC immunization. Right: Summary statistics; N = 10. c Representative flow cytometry plots of cytokine production by the Tfh cells in Fig. 4b. d Representative flow cytometry plots of Chat-GFP expression in isolated Tfh cells cultured in vitro for 16 h with either no stimulus or the indicated stimuli as in Fig. 4g. Data in Extended data Fig.4b are the mean ± SEM. P value of ****<0.0001 was determined by two-sided Wilcoxon matched-pairs test. Exact P values are reported in the Source Data.
