Extended Data Table 1 List of antibodies used for flow cytometry and functional analysis

From: Lymphocyte-derived cholinergic circuits modulate germinal center output and B cell activation

Antigen	Fluorochrome/detection	ID/Clone	Supplier	Supplier ID#	Working Dilution
B220	BUV737	RA3-6B2	Thermo Fisher Scientific	367-0452-82	400
IgD	BV510	11-26c.2a	BioLegened	405723	200
CD45	APC	30-F11	BioLegened	103112	400
CD19	BUV395	1D3	BD Biosciences	563557	400
CD3	BV785	17A2	BioLegened	100232	200
NK1.1	PerCP-Cy5.5	S17016D	BioLegened	156526	200
CD93	APC	AA4.1	BioLegened	136510	100
CD23	PE-Cy7	B3B4	BioLegened	101614	200
CD21/CD35	PE	7.00E+09	BioLegened	123410	200
CD43	BV605	S7	BD Biosciences	747726	100
CD5	APC	53-7.3	BioLegened	100626	300
CD4	Pacific Blue	RM4-5	BioLegened	100531	200
CD8α	APC	53-6.7	BioLegened	100712	400
CD43	PE-Cy7	S11	BioLegened	143210	200
GL7	PE-Cy7	GL7	BioLegened	144620	200
OX40	PE	OX-86	BioLegened	119409	100
CD150	BV421	TC15-12F12.2	BioLegened	115925	100
CD40L	PE	SA047C3	BioLegened	157004	100
IL-4	PE	11B11	BioLegened	504104	50
IL-21	APC	S20017B	BioLegened	122203	50
CD38	PerCP-Cy5.5	90	BioLegened	102722	200
CD95	PE-Cy7	Jo2	BD Biosciences	557653	200
CD86	BV605	PO3	BioLegened	105125	200
CXCR4	PE	2B11	Thermo Fisher Scientific	12-9991-82	100
IgM FCµ	BV421	AB_2651085	Jackson Immunoresearch	115-675-075	400
IgG FCγ	AF647	AB_2338904	Jackson Immunoresearch	115-605-008	400
CD45.1	BUV395	A20	BioLegened	110762	200
CD138	BV605	281-2	BioLegened	142531	100
IRF4	PE	3.00E+04	BioLegened	646403	100
CD80	BV605	16-10A1	BioLegened	104729	200
CD73	PE	TY/11.8	BioLegened	127206	100
CCR6	BV785	29-2L17	BioLegened	129823	100
CD28	PE	37.51	BD Biosciences	553297	200
TACI	APC	ebio8F10-3	Thermo Fisher Scientific	17-5942-81	100
ICOS	PerCP-Cy5.5	7E.17Gp	Thermo Fisher Scientific	46-9942-82	100
CXCR5	BV605	L138D7	BioLegened	145513	100
PD-1	PE-Cy7	RMP1-30	BioLegened	109110	200
CD4	BV510	PM4-5Sta	BioLegened	100559	200
GFP	AF488	AB_221477	Thermo Fisher Scientific	A21311	200
CD3ε	e450	ebio500A2	Thermo Fisher Scientific	48-0033-82	200
CD4	APC	RM4-5	BioLegened	100516	200
Goat α-Rabbit IgG FCγ F(ab)	AF647	AB_2632470	Jackson Immunoresearch	111-607-008	2000
c-Myc	Rabbit IgG	D84C12	Cell Signaling Technology	5605	50
CD36	APC	HM36	BioLegened	MCA2748A647	100
OX40L	PE	RM134L	Thermo Fisher Scientific	12-5905-81	100
phospho-GSK3ß (S9)	Rabbit IgG	D85E12	Cell Signaling Technology	5558	50
phospho-CREB (S133)	Rabbit IgG	87G3	Cell Signaling Technology	9198	50
Chrm2 (M2)	Rabbit IgG	AB_2039995	Alomone Labs	AMR-002	50
Chrm4 (M4)	Rabbit IgG	AB_11219338	Alomone Labs	AMR-004	50
Chrna9 (N9)	Rabbit IgG	AB_2756678	Alomone Labs	ANC-019	50
phospho-ERK1/2	Rabbit IgG	D9E	Cell Signaling Technology	4060	50
phospho-tyrosine	BV421	pY20	Thermo Fisher Scientific	48-5001-42	50
CD69	Pacific Blue	H1.2F3	Thermo Fisher Scientific	48-0691	200
Activated Caspase-3	BV605	C92-605.r	BD Biosciences	570538	100
*Functional antibodies*
α-CD16/32 FcR block		2.4G2	CyTek Bioscience	70-0161-M001
α-IgM F(ab’) ₂		AB_2338469	Jackson Immunoresearch	115-006-020
ICOS		C398.4A	BioLegend	313502
α-CD3		17A2	BioLegend	100201
α-CD28		37.51	BioLegend	102101
α-CD40		1C10	BioLegend	102802

This table lists antibodies used for surface and intracellular staining prior to flow cytometric analysis. Included are the target antigen, fluorochrome or detection method, clone or ID, supplier, and catalog number, and working staining dilution. Antibodies for functional assays are also included.

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Extended Data Table 1 List of antibodies used for flow cytometry and functional analysis

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