Extended Data Fig. 8: Additional data for genetic interactions between ADAR and PKR and mechanisms of ADAR dependence.
From: Tumor-derived IFN triggers chronic pathway agonism and sensitivity to ADAR loss
a, Waterfall plot of ADAR dependence from Project DRIVE, colored by PKR RNA expression levels. b,c, Phosphorylated PKR (p-PKR) levels in b and phosphorylated eIF2α (p-eIF2α) levels in c assessed by western blots in cell lines transduced with Dox-inducible ADAR shRNAs. Similar results were obtained in two additional experiments for SW1990 as in b and for MDA-MB-468 and HSC4 as in c. Blot images are cropped to show the relevant bands, and molecular mass markers are indicated (in kD). See Source Data for the uncropped western blots. d, Volcano plot depicts transcriptional changes following ADAR depletion in SW1990 cells (n = 2 biologically independent replicates for both control and Dox-treated samples). Dotted lines illustrate the cutoff for dysregulated genes with log2 (fold change) >1 and P < .001. P value is calculated as described in Methods. e, Heatmap depicts transcript-level changes of individual ISG and cytokine genes.
