Extended Data Fig. 4: Immunoglobulin production of different B cells from tumor-bearing mice.

(a,b) λ chain of IgA, IgG1, IgG2a, IgG2b, IgG3, IgM, and IgE produced by different B cells stimulated with lipopolysaccharide (5 μg/ml) for 48 h (n = 6 biologically independent samples) (a) or from mouse serum (n = 8 biologically independent animals) (b) were detected by CBA. (c,d) κ chain (c) and λ chain (d) of IgA, IgG1, IgG2a, IgG2b, IgG3, IgM, and IgE from MMTV-PyMT or WT mouse serum were detected by CBA (n = 6 biologically independent animals). (e) Quantitation of IgG using IgG1, IgG2a, IgG2b, IgG3 ELISA kits for normal and 4T1 tumor-bearing mouse serum (n = 6 biologically independent animals). (f) Quantitation of IgG using ELISA kits for MMTV-PyMT or WT mouse serum (n = 6 biologically independent animals). (g,h) Quantitation of IgG from different LNs (g) or 4T1 tumors (h) (n = 8 biologically independent animals). (i) λ chain of IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, and IgE produced by B cells from peripheral blood (i), spleen (k), and bone marrow (m) with 5 μg/ml lipopolysaccharide stimulation for 48 h were detected by CBA (n = 6 biologically independent animals). (j) κ chain of IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, and IgE produced by B cells from peripheral blood (j), spleen (l), and bone marrow (n) with 5 μg/ml lipopolysaccharide stimulation for 48 h were detected by CBA (n = 6 biologically independent animals). Unpaired two-tailed Student’s t-tests. Data are plotted as mean +/− s.d., which are representative of three independent replicates.