Extended Data Fig. 9: High serum anti-HSPA4 IgG is positively correlated with high tumor cell HSPA4 expression and LN metastasis in breast cancer subjects. | Nature Medicine

Extended Data Fig. 9: High serum anti-HSPA4 IgG is positively correlated with high tumor cell HSPA4 expression and LN metastasis in breast cancer subjects.

From: Tumor-educated B cells selectively promote breast cancer lymph node metastasis by HSPA4-targeting IgG

Extended Data Fig. 9

(a) Representative images of negative, weak, median and strong expression of HSPA4 in tumor tissues from subjects with invasive ductal carcinoma (IDC). Scale bars, 50μm. (b) Schematic illustration for the detection of serum anti-HSPA4 IgG levels by enzyme-linked immunosorbent assay (ELISA). (c,d) Representative immunohistochemistry images (c) and comparison (d) of SDF1α expression in benign LNs from breast cancer subjects with (LN + , n = 56 biologically independent subject samples) or without (LN-, n = 52 biologically independent subject samples) LN metastasis. Unpaired two-tailed Student’s t-tests. (e) Immunofluorescent analysis of αSMA and SDF1α expression in LNs from subjects with IDC. Scale bars, 50μm. (f) Representative immunohistochemistry images of HSPA4, CXCR4 expression in tumors and SDF1α expression in LNs from three subjects with IDC. Scale bars, 50μm. Data are representative of three independent replicates for a,c-f.

Back to article page