Extended Data Fig. 1: B cell profiles in peripheral blood, spleen and bone marrow of tumor-bearing mice.

(a) Immunohistochemistry staining of B cells with anti-CD19 antibody in DLN, NDLN and NLN. Scale bars, 500μm. (b) Proportions of CD4 + T cells, CD8 + T cells, dendritic cells (DCs, Ia/e + CD11Chi) and macrophages (CD11bhi F4/80 + ) were detected by flow cytometry in DLNs collected from 0 to 28 days after 4T1 tumor inoculation. (c) The proportions (left) and absolute numbers (right) of B cells in lymph nodes after EMT6 inoculation are shown in linear graph (n = 4 biologically independent animals for each time point). (d) Proportions of B cells (CD19 + ) within mononuclear cells were detected in peripheral blood (d), spleen (h), and bone marrow (l) at day 21 after tumor burden (n = 6 biologically independent animals). (e) Proportions of B cells (CD19 + ) within lymphocytes were detected in peripheral blood (e), spleen (i), and bone marrow (m) at day 21 after tumor burden (n = 6 biologically independent animals). (f) Absolute numbers of B cells (CD19 + ) were detected in peripheral blood (f, per 200 μl volume), spleen (j), and bone marrow (n, per one lower limb) at day 21 after tumor burden. (g) B cell subsets in peripheral blood (g), spleen (k), and bone marrow (o) were detected at day 21 after tumor burden (n = 6 biologically independent animals). Unpaired two-tailed Student’s t-tests for d-o. Data are plotted as mean +/− s.d., which are representative of two (b-o) and three (a) independent replicates.