Extended Data Fig. 4: Cytokine responses to antigen stimulation of PBMCs.
From: Identification of preexisting adaptive immunity to Cas9 proteins in humans
a, Frequency of spots detected when 5 × 105 PBMCs were applied to wells of an ELISpot plate and challenged with each different antigen; the error bars represent the s.d. (n = 18). Significance was measured using a paired Student’s t-test. b, Frequency of T cells that were positive for different cytokines on antigen stimulation, as detected by ICS (n = 18). The black bars indicate the mean percentage of T cells positive for each cytokine (n = 18). *P < 0.05, **P < 0.01, ***P < 0.001, paired Student’s t-test. Each dataset was tested for significance against the unstimulated control.
