Extended Data Fig. 8: Effect of celastrol on mice with heterozygous deletion of Il1r1.
From: IL1R1 is required for celastrol’s leptin-sensitization and antiobesity effects
a, Hypothalamic Il1r1 mRNA levels in Il1r1+/+ and Il1r1+/– mice determined by qPCR. n = 7 mice for each group. Values indicate averages ± s.e.m.; P = 0.0009, determined by two-tailed Student’s t test. b–g, Il1r1+/+ and Il1r1+/– or Il1r1–/– mice (with average body weight of 37–40 g) were fed on a chow diet and treated with vehicle or celastrol (100 μg/kg body weight, i.p., once a day) for 7 d. n = 7 mice for each group. b, Body weight. Il1r1+/+–Veh vs. Il1r1+/+–Cel, P = 0.0005; Il1r1+/––Veh vs. Il1r1+/––Cel, P < 0.0001; Il1r1–/––Veh vs. Il1r1–/––Cel, P = 0.753. c, Percent body weight change during treatment. Il1r1+/+–Veh vs. Il1r1+/+–Cel and Il1r1+/––Veh vs. Il1r1+/––Cel, P < 0.0001; Il1r1–/––Veh vs. Il1r1–/––Cel, P = 0.576. d, Average daily food intake during treatment. Il1r1+/+–Veh vs. Il1r1+/+–Cel, P = 0.001; Il1r1+/––Veh vs. Il1r1+/––Cel, P = 0.002; Il1r1–/––Veh vs. Il1r1–/––Cel, P > 0.99; Il1r1+/+–Cel vs. Il1r1+/––Cel, P > 0.99; Il1r1+/––Cel vs. Il1r1–/––Cel, P = 0.02. e, Plasma leptin levels after 7 d of treatment. Il1r1+/+–Veh vs. Il1r1+/+–Cel, P = 0.03; Il1r1+/––Veh vs. Il1r1+/––Cel, P = 0.04; Il1r1–/––Veh vs. Il1r1–/––Cel, P > 0.99; Il1r1+/+–Cel vs. Il1r1+/––Cel, P > 0.99; Il1r1+/––Cel vs. Il1r1–/––Cel, P = 0.06. f, Six-hour fasting blood glucose levels after 7 d of treatment. Il1r1+/+–Veh vs. Il1r1+/+–Cel and Il1r1+/––Veh vs. Il1r1+/––Cel, P < 0.0001; Il1r1–/––Veh vs. Il1r1–/––Cel and Il1r1+/+–Cel vs. Il1r1+/––Cel, P > 0.99; Il1r1+/––Cel vs. Il1r1–/––Cel, P = 0.0002. g, Plasma IL-1β levels after 7 d of treatment (P > 0.99, between each group). Values indicate averages ± s.e.m. P values were determined by two-way ANOVA with Bonferroni’s multiple-comparisons test. *P < 0.05, ***P < 0.001; n.s., not significant (P > 0.05).
