Extended Data Fig. 9: IL1R1 interacts with LepRb.
From: IL1R1 is required for celastrol’s leptin-sensitization and antiobesity effects
HEK 293 cells were transfected with plasmids expressing LacZ, human IL1R1 (hIL1R1), Flag-tagged LepRb, or hIL1R1 and Flag-tagged LepRb together. The experiments were repeated two times with similar outcomes. Representative immunoblots depict the immunoblotting results for IL1R1 and Flag in the Flag immunoprecipitates (top). The expression levels of IL1R1 and HSP90 in the input total-cell lysates are shown in the lower panels.
