Extended Data Fig. 1: Microarray analysis of the hypothalamic transcriptome.
From: IL1R1 is required for celastrol’s leptin-sensitization and antiobesity effects
a, Flow chart for microarray analysis. b, Heat map depicting the average log2 FC (Cel/Veh) for the 38 upregulated genes and 15 downregulated genes in pattern 10 and pattern 17 from Fig. 1h. c, Heat map showing clustering of 74 upregulated genes and 29 downregulated genes from limma F-test analysis. d, GO pathways significantly enriched in 74 upregulated genes from limma F-test analysis. e, log2-transformed fold change (log2 FC) in the nine genes present in the GO pathway regulation of inflammatory response in DIO mice after 6 h, 1 d, and 4 d of vehicle or celastrol administration. n = 4 mice (6 h, 1 d) and n = 3 mice (4 d). Values indicate means ± s.e.m. f, Heat map showing log2-transformed expression of the nine identified genes present in the GO pathway regulation of inflammatory response in DIO, lean, and db/db mice at 6 h, 1 d, and 4 d. g, Geometric mean of the fold change values at 1 d and 4 d for the identified genes in DIO, lean, and db/db mice. h,i, Hypothalamic mRNA levels for the identified genes in DIO mice treated with vehicle or celastrol for 1 d (Il1r1, P = 0.0002; Ada, P = 0.0001; Nfkbia, P = 0.01; Ptgs2, P = 0.003; Tgm2, P = 0.0002; Zfp36, P = 0.01; n = 6 mice for each group) (h) and 4 d (Il1r1, P = 0.01; Ada, P = 0.03; Nfkbia, P = 0.01; Ptgs2, P = 0.009; Tgm2, P = 0.007; Zfp36, P = 0.02; n = 4 mice for each group) (i). Values indicate averages ± s.e.m. P values were determined by two-tailed Student’s t test in h and i. *P < 0.05, **P < 0.01, ***P < 0.001.
