Extended Data Fig. 8: Different pattern of activated CD4⁺ T cells in patients with autoimmune diseases versus influenza infection.

a, In Fig. 2h, t-SNE visualization and unsupervised clustering of activated (CD38⁺) memory (CD45RA⁻) CD4⁺ blood T cells in indicated participant groups and gluten tet-positive (tet⁺) cells of patients with UCeD are shown. One cluster containing 75% of tet⁺ cells (cluster 1) from seven patients with UCeD and one cluster dramatically upregulated in subjects with influenza infection (cluster 2) are color coded in Fig. 2h. The prevalence of activated CD4⁺ memory T cells belonging to cluster 1 and cluster 2, respectively, for each indicated participant group is shown. Here, we randomly selected n = 5 distinct samples per participant group, except for the gluten challenge group for which we only had n = 4 distinct samples with sufficient cells. The median frequency and the interquartile range are shown, as well as the whiskers, showing the largest and smallest values below 1.5 times the interquartile range. The single data points depict outliers. b,c, Heat map of the indicated proteins in cluster 1 and cluster 2 with absolute expression (staining intensity) (b) and versus CD4⁺ blood T cells depicted as the log₂ fold change of the grand mean of donor marker intensity (c).