Extended Data Fig. 7: Assessment of NEUN+ cells and body weight in ZFP-treated mice.
From: Allele-selective transcriptional repression of mutant HTT for the treatment of Huntington’s disease
a–d, Representative whole-brain NEUN and ZFP immunostaining images from 6–12-month-old WT (a,b) and zQ175 het (c,d) cohorts injected with AAV2/1 + 2 ΔDBD in the left hemisphere and either AAV2/1 + 2 ZFP-B (a,c) or ZFP-D (b,d) in the right hemisphere; scale bar, 200 μm. ZFP, green; DAPI, blue; NEUN, red. Expanded regions are shown at left (labeled 1) and right (labeled 2); scale bar, 50 μm. Similar results were replicated in at least one independent experiment. e–h, Quantitation of NEUN+ cells (e,f) and NEUN intensity (g,h) in WT (left panels) or zQ175 het (right panels) mice injected with AAV2/1 + 2 ZFP-B (e,g) or ZFP-D (f,h). e–h. n = 4 mice; mean ± s.d.; two-sided unpaired t-test with Welch’s correction. i,j, Weekly body weight assessments for ZFP-B- (i) and ZFP-D- (j) treated 6-month-old WT and zQ175 het mice until 12 months of age. WT female (red circles, n = 6); WT male (navy triangles, n = 6); zQ175 het female (pink squares, n = 6); zQ175 het male (blue inverted triangles, n = 6); mean ± s.d.
