Extended Data Fig. 1: Western blot analysis of ARAF overexpression in HeLa cells.
From: ARAF recurrent mutation causes central conducting lymphatic anomaly treatable with a MEK inhibitor
It demonstrates the phosphorylation status of ERK1/2, AKT, p70S6K, mTOR and p38 in HeLa cells after serum deprivation or serum deprivation followed by a short stimulation with 10% fetal bovine serum (FBS). Normalized ratios are illustrated by the panel on the bottom. The data are shown as the mean ± s.e.m. (error bars) of five independent experiments. Two-tailed unpaired t-test with 8 df. *P = 014; ****P = 7.7 × 10−5. The images were cropped for better presentation.
