Extended Data Fig. 5: Quantitative analysis of ViewHIV study.
a, Quantitation of total vDNA foci per cell observed in microscopy images. Center line and error bars represent mean ± s.e.m. numbers of vDNA foci per cell 12 h.p.i. Data in panels a–c were obtained from two independent transduction experiments, each using an independent CD4+ T cell donor. The total number of images analyzed for each condition in each panel is from left to right: n = 118, n = 26, n = 49, n = 55, n = 44, n = 43, n = 57. Significant P values relative to DMSO by unpaired two-tailed Mann-Whitney U tests are indicated by asterisks. ****P = 6.1 × 10−12. b, Quantitation of nuclear vDNA foci per cell. Center line and error bars represent mean ± s.e.m. numbers of nuclear vDNA foci per cell 12 h.p.i. Significant P values relative to DMSO by unpaired two-tailed Mann–Whitney U tests from left to right were: ****P = 1.7 × 10−11, *P = 0.026, ****P = 6 × 10−14, ****P = 4.8 × 10−9, ****P = 2.8 × 10−12, ****P < 1 × 10−15. c, Percent co-localization of vDNA and CA foci. Center line and error bars represent mean ± s.e.m. percentage of all vDNA foci that co-localized with CA foci at 12 h.p.i. Significant P values relative to DMSO by unpaired two-tailed Mann–Whitney U tests from left to right were: ****P = 1.2 × 10−5, ****P = 1 × 10−8, ****P = 9.5 × 10−12, ****P < 1 × 10−15.
