Extended Data Fig. 2: Therapeutic regimens in mental-stress settings and individual tumor-growth curves.

a–c, Schematic showing the timing of SD, tumor injection and chemotherapy with MTX (a). Individual tumor-growth curves (mouse per mouse) of the results regarding MCA205 fibrosarcomas (b) and TC-1 lung carcinomas (c), as plotted in Fig. 2a,b, respectively. d,e, Schematic diagram showing the timing of vaccination with dying MCA205 cancer cells, SD and live MCA205-tumor-cell rechallenge (d). Individual tumor-growth curves of Ctrl and SD mice that received the prophylactic tumor vaccine (VAC) or PBS control (e), as shown in Fig. 2c. f–g, A schematic diagram illustrating the timing of SD, tumor injection and immunotherapy with PD-1 blockade (f). Individual tumor growth curves of Ctrl and SD mice treated with anti-PD-1 or ISO (g), as shown in Fig. 2d. h–j, A schematic diagram indicating the timing of AR, tumor-cell inoculation and MTX-based chemotherapy (h). Individual tumor-growth curves of MCA 205 fibrosarcomas (i) and TC-1 lung cancer (j) in this setting, as shown in Fig. 2e,f. k, Gating strategies for determining the frequency of DCs (CD11c⁺I-A/I-E⁺), macrophages (F4/80⁺I-A/I-E^-), neutrophils (CD11b⁺Ly6G⁺) and T lymphocytes (CD3⁺CD11b^-) in CD45⁺ tumor-infiltrating leukocytes.