Extended Data Fig. 7: Analysis of the CITE-seq ADT expression.
From: Single-cell immune landscape of human atherosclerotic plaques
viSNE plots of T cells (n = 2,315 cells) clustered using the ADT data, and overlaid by (a) tissue type, or expression of canonical (c) or functional (d) T cell markers. (b) Frequency of T cell subtypes per tissue. (e) Functional surface marker expression in individual cells per tissue type. For CD8+ T cells, n = 379 plaque cells and n = 159 blood cells. For CD4+ T cells, n = 287 plaque cells and n = 1,387 blood cells. The approximate P values were determined using a two-sided Mann-Whitney test, lines indicate the mean. (f, g) viSNE plots of plaque cells (n = 1,708 cells) overlaid to indicate macrophage population (f), or expression of macrophage markers (g). (h) viSNE plot of clustered macrophages (n = 300 cells) overlaid with CD206 expression. (i) Macrophage population of the CITE-seq experiment gated for expression of CD206 and CD64.
