Extended Data Fig. 2: Technical details of single-cell analysis of patient CRUK0242.
From: Pulmonary venous circulating tumor cell dissemination before tumor resection and disease relapse
a, Consort diagram describing samples used for downstream analysis. Only patients with ≥5 PV-CTCs (n = 29) were processed through single-cell isolation (DEPArray). Single cells were not isolated from 6 of the 29 samples owing to failures during sample loading into the DEPArray machine. From the remaining 23 samples, 7 patients whose single CTCs isolated did not meet morphology criteria (Methods) were excluded. Sixteen samples were processed for WGA, and two patients whose CTCs did not show good quality GII in quality control after WGA were removed (Methods). b, Table showing cases of relapse in the patients with single PV-CTCs isolated. c, Agarose gel showing results of a quality-control PCR assay used to determine the genome integrity of each sample (n = 1). 0–4 bands determine the overall DNA integrity of each sample. DEPArray images of corresponding PV-CTC (green, cytokeratin; blue, CD45; purple, DAPI) are shown above. d, Examples of copy number profiles detected in single PV-CTCs, CECs, and WBC controls. Blue and red indicate regions of copy number loss and gain, respectively.
