Extended Data Fig. 1: Islet area in the eye is correlated with beta cell area in the pancreas and lower fasting glucose.
(a) Scatterplot of fasting glucose levels and eye islet area in db/db mice transplanted with islets in the anterior chamber of the eye and treated with AAV-Adipsin or AAV GFP (n = 5 per group). (b) Scatterplot of beta cell area and eye islet area in db/db mice transplanted with islets in the anterior chamber of the eye and treated with AAV-Adipsin or AAV-GFP (n = 5 per group). (c) Representative immunohistochemistry images of insulin (brown) performed in pancreatic sections from db/db-GFP and db/db-Adipsin mice. IHC was performed at least twice independently with similar results. Scale bars, 100 µm. (d) Quantification of beta cell area in db/db mice transplanted with islets in the anterior chamber of the eye and treated with AAV-Adipsin or AAV-GFP (n = 5 per group). Data were analyzed by two-tailed unpaired t-test. (p = 0.027). (e) Western blot for adipsin from sera of B6 WT mice injected with GFP-AAV or adipsin-AAV after 5 months (n = 4 per group) along with quantification. (f) Quantification of islet area in the eye over time in WT-GFP and WT-Adipsin mice (n = 5 WT-Adipsin, n = 6 WT-GFP). Eye imaging was repeated at least 3 times independently with similar results. (g) Reporter islets transplanted into the anterior chamber of the eyes of WT-GFP and WT-Adipsin mice were serially imaged by light microscopy. Representative images are from a single mouse in each treatment group at the indicated time points. Islets are outlined within dashed white circles. Islets are amplified inside the white box. Eye imaging was repeated at least 3 times independently with similar results. Scale bars, 1 mm. Data are expressed as mean ± s.e.m. *P < 0.05, ** P < 0.01, *** P < 0.001.
