Extended Data Fig. 4: Adipsin increases the expression of beta cell transcription factors and decreases gastrin expression.
(a,b) Representative images (a) of MAFA and insulin IF in B6 WT mice, db/db mice injected with AAV-Adipsin or AAV-GFP along with quantification (b) of MAFA + beta cells (n = 4 mice for B6 WT, n = 6 mice per db/db treatment group). Data were analyzed by two-tailed unpaired t-test. (p = 0.029). (c,d) Representative images (c) of NKX2-2 and insulin IF in the indicated groups along with quantification (d) of NKX2-2 + beta cells (n = 5 mice per group). Data were analyzed by two-tailed unpaired t-test. (p = 0.027). (e,f) Representative images (e) of gastrin and insulin IF in the indicated groups along with quantification (f) of gastrin + beta cells (n = 5 mice per group). White dashed box indicates region magnified in white panel. Data were analyzed by two-tailed unpaired t-test. (p = 0.038). Scale bars, 100 µm (a, b and c). Data are expressed as mean ± s.e.m. *P < 0.05, ** P < 0.01, *** P < 0.001.
