Extended Data Fig. 2: Sanger DNA sequencing of homozygous APOEch carrier individual.

Representative direct sequencing results of amplicon in APOE gene from control, proband, and descendants’ samples. R136 homozygous sequence is shown in upper panel from a control individual. In the middle panel, proband case is shown. R136S homozygous mutation can be observed, resulting from a change from cytosine (C) to adenine (A). In bottom panel a R136S heterozygous mutation from a descendant is identified. Data representative of n = 2 independent experiments and further validated independently with whole exome and whole genome sequencing.