Fig. 3: The L. rhamnosus blood-isolate-specific rpoB SNP occurs at the rifampin-binding site and confers rifampin resistance. | Nature Medicine

Fig. 3: The L. rhamnosus blood-isolate-specific rpoB SNP occurs at the rifampin-binding site and confers rifampin resistance.

From: Genomic and epidemiological evidence of bacterial transmission from probiotic capsule to blood in ICU patients

Fig. 3

a, Predicted structure of LGG RNA polymerase β-subunit RpoB showing the rifampin-binding site (white) with H487 of the probiotic (blue, left) and D487 of the blood isolate from Patient R1 (magenta, right). b, Rifampin susceptibility testing of blood isolates of each patient (R1–R6) compared to a probiotic isolate with no SNPs (P3-2). Bars depict the medians of three independent experiments, and error bars show the interquartile ranges. *P = 0.0021 for R1 compared to P3-2 by Kruskal-Wallis test followed by Dunn’s multiple comparisons test. The blood isolate from Patient R1 was resistant based on zone cutoffs for Staphylococcus aureus (Supplementary Table 8).

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