Extended Data Fig. 5: Diet-induced maternal obesity and prenatal androgen exposure effects on body weight and adipose tissue and liver gene expression.
From: Prenatal androgen exposure and transgenerational susceptibility to polycystic ovary syndrome
a-b, Body weight development and area under the curve (AUC) of body weight in F1, F2 and F3 adult female offspring from 3 to15 weeks of age. c, Percent lean mass normalized with body weight (grams) in F1 to F3 adult female offspring at 18-week of age. d, Subcutaneous adipose tissue mRNA expression (2-ΔΔCT) of genes involved in adipogenesis; Bmp4, Zfp423, Cebpa, Cebpb, Ppard, and Pparg of F1-F3 female offspring. (n = animals per group. F1: MatCD + Veh, n = 6, MatCD + DHT, n = 5, MatHFHS + Veh, n = 5, MatHFHS + DHT, n = 5. F2: GMatCD + Veh, n = 6, GMatCD + DHT, n = 5, GMatHFHS + Veh, n = 5, GMatHFHS + DHT, n = 1. F3: GGMatCD + Veh, n = 4, GGMatCD + DHT, n = 3, GGMatHFHS + Veh, n = 5). e, Gene expression (2-ΔΔCT) of lipid biosynthesis and free fatty acid oxidation pathways; Acaca, Fasn, Scd1, Pparg, Fitm1, Apoa1, Lxra, and Srebf1 in liver of F1-F3 female offspring. (n = animals per group. F1: MatCD + Veh, n = 9, MatCD + DHT, n = 10, MatHFHS + Veh, n = 11, MatHFHS + DHT, n = 8. F2: GMatCD + Veh, n = 10, GMatCD + DHT, n = 7, GMatHFHS + Veh, n = 10, GMatHFHS + DHT, n = 1. F3: GGMatCD + Veh, n = 9, GGMatCD + DHT, n = 8, GGMatHFHS + Veh, n = 8). F1: two-way ANOVA, Tukey’s post hoc analysis; F2 and F3: one-way ANOVA, Dunnett’s post hoc analysis. All data are presented as mean ± s.e.m. Number of mice are stated in bars of each group or in the text. Veh = vehicle; DHT = dihydrotestosterone; CD = control diet; HFHS = high-fat high-sucrose; Mat = maternal; GMat = grand-maternal; GGMat = great-grand maternal.
