Extended Data Fig. 10: Effective AAV9-encoded gene expression throughout the entire cervical spinal cord of the adult monkey after a single bolus, subpial C3 delivery of AAV9-UBI-Rpl22-3×HA. | Nature Medicine

Extended Data Fig. 10: Effective AAV9-encoded gene expression throughout the entire cervical spinal cord of the adult monkey after a single bolus, subpial C3 delivery of AAV9-UBI-Rpl22-3×HA.

From: Spinal subpial delivery of AAV9 enables widespread gene silencing and blocks motoneuron degeneration in ALS

Extended Data Fig. 10

a-c, An intraoperative photograph depicting the placement of subpial injection needle in the C3-C4 segment and rostro-caudal spread of ‘blue’ dextran (10,000 MW; 300 µl) immediately after initiation of subpial infusion (1 µl/5 sec). d-f, Expression of Rpl22 protein (anti-HA staining; green signal) in cervical spinal cord segments (C1-C8) at 48 h after mid-cervical subpial AAV9-UBI-Rpl22-3xHA delivery. g-l, Neuronal (NeuN), astrocyte (GFAP) and oligodendrocyte (Olig2)-expression of Rpl22 protein (HA+ signal) at 48 h after AAV9-UBI-Rpl22-3xHA delivery. m, n, Absence of specific staining in non-injected animals. A representative result of 2 individual non-human primates per group is shown (Subpially-injected, n = 2; Control non-injected, n = 2). DH, dorsal horn; VH, ventral horn.

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