Extended Data Fig. 4: Phenotype of SARS-CoV-2-specific CD8+ T cells.
From: Characterization of pre-existing and induced SARS-CoV-2-specific CD8+ T cells
a, t-SNE representation of flow cytometric data, which were derived from 18 convalescent SARS-CoV-2 individuals after ex vivo pMHCI tetramer-based enrichment (10-20*106 PBMCs used for enrichment), comparing SARS-CoV-2-specific CD8+ T cells by their HLA restriction (left) and expression levels of CD127, CXCR3, FOXO-1, CX3CR1, CD57, KLRG-1 and CD25 plotted on the t-SNE plot. b, Exemplary histograms depicting the expression levels of CD127, BCL-2, TCF-1 and FOXO-1 on CD8+ bulk (black) and non-naïve CD8+ T cells (grey) as well as SARS-CoV-2-specific CD8+ T cells (white) ex vivo after pMHCI tetramer-based enrichment. c, BCL-2 expression of different memory cell populations on bulk CD8+ T cells (left) and of SARS-CoV-2- and Flu -specific CD8+ T cells ex vivo after pMHCI tetramer-based enrichment of the different CD8+ T-cell subsets. Bar charts show the median with IQR. n=6 (A*01/ORF3a207-215, A*01/ORF1ab4163-417), n=12 (A*02/ORF3a139-147), n=7 (B*07/N105-113), n=3 (B*44/N322-330, B*44/ORF1ab3946-3954), n=5 (A*02/Flu-M158-66). Statistical significance was assessed by Kruskal-Wallis or Friedman rank-sum C, testing including Dunn’s multiple comparisons test.
