Extended Data Fig. 7: Extended plasma analysis using Olink proteomic assay.

a, PDCD1, CXCL9, CXCL10 normalized protein expression (NPX) in plasma of patients (n = 85) before treatment (pretreatment; round dots) and after treatment (post-treatment; triangle dots) measured with Olink immunoassay (green: patients with pathologic response, n = 64; red: patients without pathologic response, n = 21). Data for PDCD1 are missing for pretreatment samples of 9 patients (all responders) because values were below detection limit. The mean and SD are shown. P values were calculated using the paired Student’s t-test (two-sided). b, Heatmap of VEGFR2, CX3CL1 and PD-L2 NPX in plasma of patients before start of treatment. The heatmap depicts the ordered mean expression of these three genes of the 86 patients included in the OpACIN-neo cohort. The score of each patient expresses the baseline averaged z-score of the three mentioned genes mentioned beforehand which was previously normalized by DESeq2. Each column represents a different patient (green: response/no relapse; red: no response/relapse; blue: arm A; orange: arm B; purple: arm C) and rows indicate protein expression. Positive values (red) indicate higher expression and negative values (blue) indicate lower expression.