Extended Data Fig. 6: TEM of sorted B cells after immunization with eOD-GT5 or eOD-GT8.
From: Engineered immunogen binding to alum adjuvant enhances humoral immunity
(a, b) Mice were adoptively transferred with VRC01gHL B cells then immunized with fluorescent pSer8-eOD-GT5 and alum i.p. Two days after immunization, endogenous (a) and eOD-GT5+ VRC01gHL B cells (b) were sorted by flow cytometry, fixed, and sectioned for TEM imaging. 145 (a) and 153 (b) cells were analyzed, and representative images are shown. (c, d) Identical experimental conditions were used, with pSer8-eOD-GT8 as the antigen, for TEM imaging of endogenous (c) and eOD-GT8+ VRC01gHL B cells (d). 126 cells were imaged for (d) and 45 cells were imaged for (c). (e) Quantification of images from (c) and (d). Arrowheads point to electron dense alum clusters.
