Extended Data Fig. 2: Depletion of mtDNA by Cas9 does not alter bacterial composition after 30 cycles of amplification.
From: Viable bacterial colonization is highly limited in the human intestine in utero
16S rRNA V4 profiling of a subset (n = 10) of banked fetal meconium samples using different library preparation methods: gel extraction and 30 or 35 cycles of amplification, or 30 cycles combined with DASH performed on individual samples (Individual DASH) or on the library pool (Pooled DASH). a. Expansion in Enterobacteriaceae family is detected in 35-cycle amplification method, while small expansion of Pseudomonadaceae is detected post-DASH. Principal coordinates analysis of Bray Curtis distances of libraries using b. 30 cycles of amplification or c. 30 and 35 cycles of amplification, latter to provide an outgroup known to skew bacterial composition. Ellipses indicate 95% confidence intervals. All p-values were calculated using two-sided Satterthwaite’s method on Linear Mixed Effects (LME) modeling to correct for n = 10 paired samples that underwent multiple library preparation methods.
