Extended Data Fig. 9: Total HIV DNA in CD4 T cells.

Total HIV DNA in CD4 T cells. a, Total HIV DNA at baseline (pre-vaccination) and pre-ATI (post-vaccination) did not significantly differ between the vaccine versus placebo arms. Open symbols depict levels below the limit of detection of the assay and were plotted at the limit of detection, which varies between participants (1–2 copies/106 CD4 T cells). b, Longitudinal total HIV DNA values demonstrated significant decline of DNA between acute infection and pre-vaccination in both arms after approximately 2 years on ART. The total HIV DNA decline achieved borderline significance in the placebo arm between pre- and post-vaccination, which was driven primarily by the single PTC in that arm. In both arms, the total HIV DNA rose after ATI but returned to baseline values after ART resumption. Open symbols depict levels below the limit of detection of the assay and were plotted at the limit of detection, which varies between participants (1–2 copies/106 CD4 T cells). c, This sole PTC with HLAB57:01:01 was in the placebo arm. He had a delay in viral rebound and subsequent spontaneous control. His total HIV DNA declined after vaccination and did not rise above pre-vaccination values after ATI. His CD4 T cell count did not significantly decline during the study. Open symbols depict measurements from the ATI period.