Extended Data Fig. 10: Flow cytometry analyses. | Nature Medicine

Extended Data Fig. 10: Flow cytometry analyses.

From: Safety and immunogenicity of Ad26 and MVA vaccines in acutely treated HIV and effect on viral rebound after antiretroviral therapy interruption

Extended Data Fig. 10

Flow cytometry analyses. a, Representative flow plots showing the gating strategy for flow cytometry analysis of intracellular cytokine staining to determine cytokine expression by CD4 and CD8 T lymphocytes upon stimulation with vaccine-insert matched peptide pools. b, Representative flow plots showing the gating strategy for flow cytometry analysis to determine the frequency of PKH26+CFSE- target cells. Each sample was run in triplicates and the average was calculated. c, Flow cytometry gating scheme used to quantitate monocytic THP-1 ADCP is shown for representative plasmas sample collected at baseline (left) and week 50 (right) from a vaccinated individual. THP-1 cells were gated on based on FSC-A and SSC-A. Then, doublets were excluded based on low outlier events for FSC-H against FSC-A. Final histogram gating was used to determine bead positive THP-1 cells. The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity of the bead-positive cells and dividing by 104. Each sample was run in duplicate and the average was calculated.

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