Extended Data Fig. 1: Reactivity of human anti-PfCSP antibodies with different PfCSP peptides.

a, ELISA-reactivity of monoclonal human antibodies²⁰ to KQPA, NPDP, NVDP, NANP and C-CSP at the indicated antibody concentrations. Red and green lines indicate positive (2A10; ref. ¹⁹) and negative controls (mGO53⁴²), respectively. n indicates the number of tested antibodies. b, Binding strength of anti-PfCSP antibodies (n = 200; Supplementary Table 1) to the indicated overlapping peptides and C-CSP as in (a) is shown as calculated area under the curve (AUC) values based on ELISA measurements at different antibody concentrations. The frequency of reactive and non-reactive antibodies is indicated. c, t-SNE clustering-based illustration of the ELISA binding strength to the indicated peptides as determined by AUCs for all PfCSP-reactive antibodies (n = 200). d, Antibody affinities to the indicated PfCSP peptides and C-CSP measured by SPR. e, t-SNE clustering-based illustration of the affinity of PfCSP-reactive antibodies to the indicated peptides and C-CSP measured by SPR for antibodies with ELISA AUC values >5. n indicates the number of antibodies.