Extended Data Fig. 2: Ig gene usage of anti-PfCSP antibodies with different reactivity profiles and cross-reactivity of IGHV3-33-encoded antibodies.

a, IGHV and paired IGKV or IGLV gene usage of antibodies with the indicated binding profiles: NANP-specific, NANP, NVDP cross-reactive and NANP, NVDP, NPDP cross-reactive antibodies; C-CSP cross-reactive antibodies, and NVDP-specific and NVDP, NPDP cross-reactive antibodies. Antibodies using the same IGHV are indicated as solid lines around the pie charts. n in the pie chart centers indicate the number of antibodies. b, ELISA PfCSP-reactivity of monoclonal plasmablast antibodies²⁰ (n = 111) tested at 4 µg/ml (left). The PfCSP reactivity of IGHV3-33-, IGKV1-5-encoded antibodies including mAb 2541 (n = 6) was confirmed at different concentrations and is indicated as mean area under the curve (AUC; center). Red and green filled circles indicate the positive (2A10; ref. ¹⁹) and negative (mGO53⁴²) control mAbs, respectively. The binding profiles of the six PfCSP-reactive IGHV3-33-, IGKV1-5-encoded plasmablast antibodies to the indicated peptides and C-CSP are shown (right). Mean AUC values from three independent experiments are shown (center, right).