Extended Data Fig. 9: Schematic representation of sBLISS-derived double-strand end configurations.
From: Colibactin DNA-damage signature indicates mutational impact in colorectal cancer
a, Blunting of original break ends by T4 polymerase preserves the original 5’ ends, which can then be determined by sequencing using the sBLISS protocol. Mapping of sBLISS reads to the genome allows us to derive strand-specific break configurations across all genomic sites, scanning the same motif. Frequencies of break ends depend on the break end configuration and differ for blunt ends or breaks with 3’ and 5’ overhangs. b, Validation of the break end analysis in a cell line with an inducible AsiSI restriction enzyme 48. The identified break positions correspond to the known break points of this restriction enzyme 82.
