Extended Data Fig. 2: Validation of sBLISS for the detection of colibactin-associated DSB break points.

a, In contrast to infection with pks^- E. coli, Caco-2 cells infected with colibactin-producing pks⁺ E. coli displayed γH2AX expression, megalocytosis and multinucleation. Representative image of two independent experiments. b, sBLISS signal of etoposide-induced DSBs showed increased counts at TSS as compared to untreated control conditions. c, This heatmap indicates the log2 odds ratio of break enrichment in genomic region collections (FDR < 5%) as compared to the rest of the genome: untreated, etoposide-treated, pks⁺ E. coli-infected and pks^- E. coli-infected Caco-2 cells. Rows represent known genomic regions from different genome annotation collections. Etoposide treatment increased DSB enrichment in TSS-associated features, while DSBs in pks⁺ E. coli-infected cells show a rather weaker enrichment compared to untreated and pks^- E. coli-infected cells, indicating a more uniform distribution across the genome.