Extended Data Fig. 1: 3P10 does not bind GFRα1 and does not inhibit GDNF/GFRα1 or Neurturin/GFRα2 signaling.
From: Antibody-mediated inhibition of GDF15–GFRAL activity reverses cancer cachexia in mice
a, Sensorgrams of GFRAL (0.31-20 nM) binding to 3P10 (left); steady state affinity curve fit (right). RU, response unit. Data representative of three experimental replicates. b, Sensorgrams of 3P10 binding GFRAL (left) and not GFRa1 (middle). GFRa1 binds GDNF (right). Data representative of two experimental replicates. c, Western blot for total and phosphorylated ERK (pERK) from GFRAL and RET transfected 293 T cells incubated with GDNF or Neurturin and 3P10. Representative gel from three independent experiments.
