Extended Data Fig. 4: 3P10 reverses HT1080 tumor-induced tissue and body weight loss.
From: Antibody-mediated inhibition of GDF15–GFRAL activity reverses cancer cachexia in mice
a,b, Cumulative food intake after 1 mg/kg recombinant GDF15 (a) and inoculation with HT1080 tumor cells (b). n = 10 mice per group. Data represent mean ± SEM. Numbers above indicate p-values calculated by two-sided t-test. c, Body weights of non-tumor bearing (NTB) and HT1080 mice treated on day 11 with 3 mg/kg antibody, fed ad libidum without calorie restriction. Data represent mean ± SEM. n = 10 mice per group. Number above indicate p-values calculated by two-sided t-test. d, White adipose and liver weight on day 21 (refer to c). n = 5 per group. Data represent mean ± s.e.m. Numbers above denote p-values calculated by ANOVA. e, 24 hour food intake measured 1 or 2 days after antibody treatment (days 11-13 in c). Data represent mean ± s.e.m. On day 1, n = 8 for non-tumor bearing (NTB) and n = 9 for IgG1 and 3P10. On day 2, n = 5 for NTB and n = 4 for IgG1 and 3P10. f, Fat and lean mass measured on day 21 by MRI of calorie restricted, IgG1 and 3P10-treated HT1080 animals (n = 10) after tumors removed (See Fig. 3b). Numbers above indicates p-values calculated by two-sided t-test. Data represent mean ± s.e.m.
