Extended Data Fig. 8: Transcriptomic expression profiling of PHH cells at basal state.
From: Polygenic architecture informs potential vulnerability to drug-induced liver injury
a-g, Basal expression levels of mRNA were shown by the indicated signature of gene sets. Color scale and sample annotation colors were shown in the bottom right. (a) Liver signature genes1. (b) Signature of gene set characterizing liver sinusoidal endothelial cell (LSEC)2. (c) Signature of gene set characterizing hepatic stellate cell (HSC)2. (d) all drug transport proteins and proteins involved in bile transport and cholestasis (TCP, OATPs, OCT1, OAT2, CNT1, CNT2, ENT1, ENT2, MDR1, MDR3, MRP2, BSEP, BCRP, MATE1, MRP3, MRP4), mRNA coding proteins of N; (e-g) mRNA coding phase 1, 2, and 3 enzymes3. The Z-scores of log2(RPM+1) values were calculated within the indicated samples. Gene with RPM = 0 in all of the samples were excluded. PHH_1day_1/_2, PHH cells after 1 day of culturing (suffix means experimental batch), which was used for assessments of drug-induced transcriptomic change; PHH_2d, PHH cells after 2days of culturing; FLT, fetal liver tissue; ALT, adult liver tissue.
