Extended Data Fig. 7: SRP-2001 reduces expression of transgene-derived progerin in murine hearts.
From: A targeted antisense therapeutic approach for Hutchinson–Gilford progeria syndrome
a, Quantitative PCR analysis of transgene transcript levels in hearts of LMNAG/G mice receiving long-term treatment with saline (vehicle) or 60 mg/kg SRP-2001. Transcripts are quantitated relative to Hprt expression, as a fraction of all transgene-derived LMNA transcripts or relative to transgene-derived LMNC transcripts. Data are presented as mean values ± SD determined from 12 independent samples per treatment group (n = 6 males, 6 females). b, Western immunoblots of A-type lamins and smooth muscle actin (SMA) immunoprecipitated from heart tissue. c, Quantitation of A-type lamins from immunoblots expressed relative to smooth muscle actin (SMA), as a fraction of all A-type lamins and relative to LMNC. Data are presented as mean values ± SD determined from 12 independent samples per treatment group (n = 6 males, 6 females), shown in panel b. A, LMNA; P, progerin; C, LMNC; * p < 0.05; ** p < 0.01; *** p < 0.001 vs same transcript or protein in mice receiving saline only (vehicle).
